Yintoni na camera Goryaeva? Imithetho kwiiseli ngokubala

Namhlanje camera Goryaeva yenye izixhobo ezininzi ezisetyenziswayo kuxilongo elebhu. Ingasetyenziswa ukubala inani iiseli ezahlukeneyo egazini. Njengoko iyaziwa, uvavanyo igazi kubaluleke kakhulu ukuba inkqubo loxilongo kunye nonyango phantse zonke izifo.

Yintoni na camera Goryaeva?

Loo kakuhle waziwa isixhobo luncedo sele ziphuhlisiwe kwaye ecetywayo ugqirha Russian N. K. Goryaevym. camera Goryaeva, enyanisweni, kukho isilayidi yeglasi ethile nala enemingxuma ezincinane.

Kutheni le khamera? Ubukhulu becala ke unokukunceda ubone inani leeseli egazini, leukocytes ezithile kunye erythrocytes. Phela, ngoncedo yayo kunokwenzeka ukumisela kwande neemicroscope esetyenzisiweyo. Ifomula oku ngolu hlobo lulandelayo:

• Kg = (m2 - M1) / An

Kulo mzekelo:

• Kg - ilungeniselo neemicroscope;
• m2 - isikhundla ekunene womda Goryaeva camera iseli;
• M1 - ukuma komda ekhohlo;
• kunye - ubungakanani kwiseli enye, nto leyo umgangatho yaye 0.05 mm;
• N - inani leseli phakathi kwemida egumbini kulinganiswa.

Ubume ikhamera

camera Goryaev akukho nto innoe njenge isilayidi iglasi (kodwa ziyatyeba kakhulu kunesiqhelo) lahlulwe vee ezinqamlezayo ube zahlulo zithathu. Inxalenye phakathi iglasi iqulethe igridi ukubala. Inxalenye yokugqibela egumbini efanayo asetyenziswa namafutha aqholiweyo coverglass - ngaloo ndlela, umgcini kudaleke igumbi evaliweyo izithuba capillary emacaleni kwaye ngalo ngayo izaliswe ulwelo.

Njengoko kwimigca enqamlezeneyo, egumbini Goryaeva yohlulwe izikwere ezinkulu ezilinganayo-225 - ukuba ibekwe kwimiqolo elinesihlanu. 25 izikwere enkulu zahlulwe zibe ezincinci, 16 ngasinye. Ubude kwicala ngalinye encinane yembutho ngu 0.05 mm.

Amalungiselelo ukubala izinto esizicelayo

Kakade ke, kulo mzekelo, oko ubuchule kakhulu iimviwo elebhu. Kakade ke, sonke egumbini iindawo Goryaeva aqaqambe neyomileyo. Emva koko, bubomi yeglasi isiciko ukuze akwazi ukubona iringi kuphawu eliqaqambileyo. Kubalulekile ukuba egumbini wayengekho amaqamza, njengoko oku sinokuwagqwetha iziphumo zohlalutyo.

Ngokuqhelekileyo, ukuze ubale inani corpuscles, wezenzi ezahlukeneyo zisetyenziswa ezahlukeneyo ngamnye egazi. Umzekelo, kusetyenziswa ukubala erythrocytes 0.9% solution sodium chloride. -In vitro ukuxuba 8 ml isisombululo ityuwa kunye 0,02 ml egazini. Ngenxa yoko, laboratory dilutes igazi izihlandlo 400. Ngamanye amaxesha ukuzala ingaba nkulu.

Ukuze ukuba zibale inani leukocytes, kuyimfuneko ukuxuba 0.4 ml acetic acid (ukuthatha-3% okanye 5% isisombululo) kunye 0,02 ml igazi.

Emva kokuba izithako ezixutywe kwityhubhu uvavanyo, usebenzisa pipetting okhethekileyo ukuzuza imali encinane mxube kunye egumbini ukugcwaliswa kakuhle i akhawunti (njenge, elinye okanye amabini amathontsi wanele).

Indlela ukubala inani leeseli igazi?

Ukubala kwe egazi abomvu Goryaeva egumbini lwenziwa kwiindawo zembutho ezintlanu ezinkulu, nto leyo ilingana asibhozo amancinci. Ukwenzela ukuphepha iimpazamo ngenxa ukwabiwa ngokungalinganiyo iisampulu igazi, khetha izikwere amalungiselelo kwikonw ngekona. ingqalelo ekhethekileyo ihlawulwe iiseli ebekwe ngeenxa zonke iikona - zithathwa iiseli ezibomvu ekhohlo phezulu beendonga, kodwa musa ukuthatha ingqalelo abo kwi emazantsi kunye nemigca tye.

Ukuze kubonwe inani iiseli ezibomvu milliliter igazi ngalinye, inani leseli kwizikwere ezintlanu ezinkulu iphinda phindiweyo ngo-20 000 (xa exutywe izihlandlo 400).

Leukocytes egumbini Goryaeva ngenye indlela. Apha kuyimfuneko ukubala inani izinto nekhulu ubuncinane izikwere enkulu. Inani ngenxa yahlulwe ngo-1600, yaye emva koko iphinda phindiweyo ngo-4000, yaye emva koko ngo-20 (ukuxutywa).