Izinga isampula Thymol, kunye nexabiso diagnostic

Kwezinye izifo ukuxilonga ekuqulunqeni kusetyenziswa iisampuli ludaka. Omnye wabo - test turbidity thymol, ecetywayo 1944 Maklaganom. Oku kusekelwe notshintsho uzinzo colloidal kweziyi serum igazi izifo ezibandakanya dysproteinemia.

Ngokuqhelekileyo, iiproteni igazi kwi ukuxhathisa karhulumente ophezulu. Xa ukutshintsha albumins ngokulinganayo amaqhezu kunye globulin uzinzo colloidal kweziyi lifinyele. I elisezantsi oko, iiproteni ezininzi sivete kwaye kwenze ngokongeza thymol reagent.

Xa wenza uvavanyo reagent lisetyenziswa isisombululo utywala of thymol in veronal buffer okanye Antipatris buffer. Ekugqibeleni, imichiza abasabela akucaci. Noko ke, thymol ukuvavanya ukuba masihambelane ngokucacileyo ngomfanekiso bezonyango sifo, zisenzeka kunye dysproteinemia. Kulula nendawo kwaye eningaxhamlekanga ngalo, ngoko kuqhubeka ukuba ngokubanzi kusetyenziswa kwiimvavanyo kwegazi ezinto eziphilayo.

Xa uzibekela kwisampula saline yaziswa serum nomonde, ke wongezelela ukuya reagent. Ukuba Kulungile uvavanyo thymol kunye ilahleko kancinane kakhulu kukubola iiproteins kunye clouding kancinane umxube reaction, xa isicombululo kwimeko dysproteinemia waba turbid kakhulu. The degree of turbidity kuxhomekeke iqondo iimpawu colloidal kweziyi. Isiphumo isampuli ukunyuka nge ukuncipha albumin kunye nokwanda beta-kunye gamma-globulin.

Turbidity ilinganiswa analyzer kwemichiza okanye photoelectrocolorimeter. I-reference isisombululo esetyenziswa njenge barium chloride yoxinaniso ethile.

Okubaluleke kakhulu kwezonyango, olu phando kwi-hepatitis, collagen kunye nezinye izifo ebandakanya dysproteinemia - ukunyhashwa umlinganiselo iiprothini yintloya. Kuba amanxeba hepatic uphawuleka ephakamileyo uvavanyo hepatitis thymol. Liyana ukusuka iiyunithi-0 ukuya ku-4. Xa hepatitis, iba elincumisayo kwiveki phambi senyongo. Kwezinye iimeko, ukuze zinongezele isampuli kwiiyunithi-20 okanye ngaphezulu. Ngamazinga aphezulu kufuneka yayiphindwa isampuli exutywe 1: 1 kunye serum ze isigulane kwaye isiphumo linyuke amaxesha-2.

Ukuseta isampuli ayikho hemolyzed serum ezifanelekileyo. Xa hemolysis, ukutshatyalaliswa iiseli ezibomvu, ukuba bomvu. Kulo mzekelo, isampuli iphezulu kakhulu. Uhlalutyo kufuneka siphindwe emva kokuba ucaphule omtsha igazi emthanjeni.

Zabaxwa test thymol ukuba serum lipemic (lipemic), turbid ngenxa yobukho kuwo of lipids (chylomicrons). Laboratory, uqhuba uvavanyo kunye serum kufuneka endaweni ukwenza ulawulo ulawulo-saline yi-serum nomonde exutywe saline.

Ukunqanda igazi chylous kwizifundo kwemichiza kufuneka uthathe ngqongqo ungatyanga. Kuya kufuneka zisiwe kwilabhoratri kwisithuba iiyure ezi-2 emva kokuba yokuqokelela. Xa agcinwe efrijini serum ngokufanelekileyo imveliso sample ubuninzi kwiintsuku ezisi-7.

Leyo ethetha test thymol kwalo? Ingcaciso ngayo ngolu hlobo lulandelayo: Ukuba iphezulu kakhulu kunesiqhelo, kunokwenzeka ukuba bacinge isifo kwesibindi (hepatitis okanye ukuqina), isifo sezintso of elibaleka nephrotic syndrome, izifo ezicwangcisiweyo ezifana rheumatism, isifo samathambo, scleroderma. Enye Isalathisi akunakwenzeka ukuba wenze isifo. Thymol kufuneka ihlolwe kunye nezinye izifundo. Ukuba urhanela isifo kwesibindi ngexesha elifanayo kuyimfuneko ukwenza ubuncinane zovavanyo kwemichiza kwi lilonke kwaye serum ngqo yibilirubin, transaminase, cholesterol, phosphatase obrak, zinc sulfate okanye sublimate isampula.

impilo elungileyo kuwe!