Vitrification ngeembumba kwi abathwali ngamnye

Kweli nqaku siza kuthetha ngayo ingqiqo njengoko vitrification imibungu ezinjalo. Dr Kuwayama Masashige kokusungulwa le ndlela kriotopah nkqu kunyaka ngonyaka. Umntwana wokuqala wazalwa ngenxa ngeembumba vitrification ngo-2003. elingama kweqanda yanyuswa nge-98 ekhulwini.

Isiqingatha seentombi abafumana IVF, imibungu ahlale. Kubo kubanjwe cryopreservation evumela ukuba izigulane ukuba wonge imali. Emva zonke unfreeze kwaye idlulisele ngeembumba kulula kakhulu kunokuba bazenze inkqubo vitro kwakhona. Kananjalo uhlobo inshorensi xa umfazi ukuba akakhulelwanga. Cryopreservation umntu inzuzo ecacileyo - nokuthintela ukufa ngeembumba kwezoqoqosho wahlala emveni kokuba protocol.

ontogeny

Ukuze uphuhliso zesimo kumzimba, okanye ontogeny, yaba umva ukususela kwixesha ukuchumisa iphela kunye nokufa kwakhe. Esi sindululo into eqhubekayo ngexesha yaye inophawu ngokusisigxina. Kwaye asikwazi ukuba amise okanye isantya lwalo lokukhawulelana nophuhliso. Kodwa ngokwendalo kukho ukuxolelwa. Ezi zityalo, ezingenamqolo kunye nabanye ezinomqolo ezinesiqalelo ukuba amaqondo obushushu aphantsi, musa ukubonisa iimpawu uphawu eziphilayo.

Yintoni silele?

Vitrification ngeembumba kuxoxwa ngezantsi. ixesha ngamanye ezolileyo kuthiwa sokuHayibhaneyitha. Umzekelo, izilwanyana ezininzi asinde amaqondo obushushu zaseSiberia kufikelela izidanga -90, kwaye emzimbeni phantse ngokupheleleyo. Xa ukufunda eli xesha ontogenesis kwindalo kukho umbuzo malunga kukusetyenziswa amaqondo aphantsi ukuba uphazamiseko kwenxalenye kwaye ingathityazwa ngayo ekusebenzeni yezinto eziphezulu ezinomqolo, kuquka abantu.

cryopreservation

Cryopreservation - yinto indlela ephumelelayo zokumiswa iinkqubo zebhayoloji kwizisele kukungakhuseleki kwiqondo lobushushu eliphantsi. Oku kugcina umsebenzi ubomi iiseli ngexesha ukufudumeza. Ngokuthandwa, le ndlela na enasilela umbungu vitrification. 1 kriotop (krionositel enombhalo) iqulathe ukusuka ngeembumba-1 ukuya ku-3.

Ngokomzekelo, xa bekusenziwa inkqubo ezifana IVF, isiphumo ilungileyo uthathwa ukuba ihamba em- zesibeleko ayikho iimbumba ngaphezu ezimbini. Imibungu kwizinga eseleyo ukuba cryopreserved ukusetyenziswa kwixesha elizayo. Kananjalo isicelo re-IVF emva kwexesha, ukuba le nkqubo iya kubonisa umphumela elibi. Ekubeni ezinjalo iinjongo baqhubeka iimbumba vitrification kwi abathwali ngamnye.

Kwezinye iimeko, ngumkhenkce zonke iimbumba. Kuba namankazana abo ovarian syndrome hyperstimulation ngexesha superovulation induction lwenziwa kakhulu. Ngubani na Kuyanconywa qhwa? Izigulane enesifo somdlavuza, ngokukodwa phambi kokuba ichemotherapy inkqubo okanye radiotherapy. Idatha koko zisiwe iimbumba kuloo ndawo ibinomthwebeba zesibeleko. Owayevunyelwe bonisa wonke ubani ithuba ukukhulelwa emva IVF ngenxa yesizathu esithile base-. Oku kunokuba polyp endometrial, azanelanga ubukhulu endometrial kwixesha transfer ezicwangcisiweyo, ukopha kakuhle.

izigaba qhwa

Imibungu zaye emkhenkceni kwizigaba ezahlukeneyo:

• iqanda (elilodwa);
• uwaphule imibungu isigaba;
• blastocysts.

Okwangoku, kukho iindlela ezimbini imibungu amazinyo.

qhwa olucothayo

Vitrification ngeembumba oluqhutywa phaya neGauteng kade. Loo indlela yaphakanyiswa kwi 70s kunye enye iindlela zokuqala classical imibungu amazinyo. Oku kusekelwe lungapholi kade ngesantya esingaguqukiyo rate. Xa umbungu zigcinwe nitrogen engamanzi.

Kodwa ke, kufanele kuphawulwe ukuba yovalo okucothayo isisombululo cryoprotective ice ngemikroskopu ziikristale kwakheka leyo echaphazela kakubi kwiiseli ze mbungu. Oku kunokubangela buso okanye ngokupheleleyo ukutshatyalaliswa izinto eziphilayo phezu ukufudumeza. Inani ngeembumba zidluliselwe ngexesha qhwa kade kunye thawing, malunga nama-70 ekhulwini ngempumelelo.

vitrification

Emva 2010, waqala ukusebenzisa indlela entsha nolusebenzayo ngakumbi cryopreservation - vitrification. Xa sithelekisa indlela lwangaphambili, le yindlela super-fast ngumkhenkce izinto eziphilayo. Kakhulu ndandingohlukani iimbumba emva vitrification PGD (diagnosis).

Xa usebenzisa le nkqubo isisombululo cryoprotective, apho iimbumba zibekwe aluyiyo ice ziikristale ngexesha amazinyo. Ngenxa yoko, kunciphise wean amathuba mbungu. Le yiprayorithi yalo ndlela kuphela indlela yovalo, kodwa izinga ukusinda imibungu emva thawing. Ngokutsho manani, inani lokusinda iimbumba emva kwenkqubo vitrification ayikho ngaphantsi kwama-95 ekhulwini.

Kwenzeka ntoni emva thawing?

Emva thawing imibungu musa kwahluka ngeembumba eziqhelekileyo. Bayakuthatha kakuhle iingcambu kunye nokukhula. Ngokutshiswa kwezinto zonke iimbumba phantsi le hatching uncedo. Ekuqhubeni lo msebenzi umaleko komhlaba umbungu yahlulwe laser umqadi ekujikeni olifunayo kwaye ikhuselekile. Oku kube lula umbungu ngaphandle iqokobhe kwaye kwandisa amathuba abohlukeneyo ngempumelelo kwi isibeleko.

Yokuvala imibungu kuvumela yokugcina ixesha elide. Le nkqubo iindleko-kakuhle, ngenxa yokuba ixabiso kulondolozwe, thawing kwaye sele betyale zonke umbungu kwi isibeleko lingaphantsi kwe yinkqubo yesibini neqanda.

Vitrification ithathwa njenge lenguqu ngezigaba apho isisombululo abandayo ngaphantsi olupholisa ngezantsi lobushushu yeglasi yenguqu. Ngelo xesha nye ihlala isakhiwo amorphous balegilasi xa ingenalo ubisi kwaye ufumana umgangatho ofanayo eziqinileyo crystalline. Ngenxa yoko, bobabini iiseli ophilileyo, kwaye nkqu yonke umbungu uguqulwe "iglasi". isakhiwo yiglasi ye ulwelo ifunyenwe phantsi vitrification ngenxa lungapholi ngamandla, ngamanye entropy le ulwelo reduced kwithuba elifutshane ixesha kunokuba kuyimfuneko isakhiwo entropy njengekristale.

Ngamagama alula, ulwelo akuthethi ngumkhenkce ukuba indlela yayo entropy ukuba entropy nomkhenkce. Kodwa ukuze vitrified kakuhle esiphilayo, kuyimfuneko kukuzuza izinga lobushushu ukuhla kwe ≈ 108 ° C / min., Yaye akunakwenzeka ngokomsebenzi, ngokuba engamanzi cryogenic isicelo akukho esaneleyo kule iqondo lobushushu, kwaye ayinakusetyenziswa vitrifitsiruemy isisombululo zibe ngumqulu incinane umthamo kweqanda. Oku konke icebisa kunye vitrification imibungu. Yintoni na, ke ngoku ngaphezulu okanye ecacileyo ngaphantsi.

Izazinzulu akwazi ukungqina ukuba ukunyuka ngoLwesithathu ngumkhenkce cryoprotectants kukuvumela ukuba ngokukhawuleza ukuthoba isantya ebandayo. Ngenxa yoko, kwi ukuxinana-10% propylene ethers nesantya etilengligolya liyehla, kwi-40 ekhulwini lexinene vitrification kunokwenzeka kwi nokuphola kumyinge we-10 ° C / min, kwaye kwi-60% ngereyithi kusithwa 50 ° C / min. Kodwa ukwanda lexinene cryoprotectants, esiwa ngoLwesithathu, ukwandisa ifuthe lazo elibi qhwa of biomaterials. ukuqanda Slow kudale sibe namanzi ebandayo kwindawo kokuphilayo eziphilayo elementi intracellular. Loo imeko kwenzeka ngenxa kwiiseli emzimbeni kakhulu kunye ukubonakala ngumkhenkce extracellular.

Ngako oko, emva kokufumana izakhiwo vitreous phula iikhemikhali kunye neenkqubo ebonakalayo emzimbeni. Ngaphandle kwento yokuba vitrification ezingekazalwa (oko kukuthi, iye yachazwa iinkcukacha ngasentla) kuyinto nzima inkqubo emzimbeni, izinto le sakhiwo inokufumaneka kubomi bethu bemihla ngemihla (iglasi, lesilicon, njalo njalo).

Vitrification imibungu: reviews

Le ndlela uqokelela ingxelo kuphela HIV. Inkqubo vitrification kunokwenzeka. Kodwa ezininzi iimpawu zikumanqanaba ahlukeneyo kuphuhliso iilebhu IVF. Vitrification - ayikho indlela entsha cryopreservation aphilayo. Nguye inyathelo lokugqibela yovalo kade. Namhlanje, abafazi abaninzi bayakwazi ukuba usana ngenxa uphuhliso lwezenzululwazi.

iziphumo

Ngenxa umsebenzi izazinzulu ezininzi vitrification iya kwenziwa ngaphandle kokusebenzisa siqandisi esetwe iyabiza kwaye usebenzisa izixhobo elula iliso abawasebenzisayo. Ngenxa yoko, indlela lula kunye nokuphucula isiphumo. Nangona inkqubela enkulu cryopreservation, ukuphunyezwa kwe ulondolozo olufanelekileyo eziphilayo kwiqondo lobushushu eliphantsi namhlanje akunakwenzeka.