Linked immunosorbent kulingwa: isicelo neempawu

Linked kulingwa immunosorbent - ukufunda elebhu, olusekelwe phezu ekuchongweni izakhiwo "iantigens-lomzimba 'kunye iileyibheli ezikhethekileyo enzymatic ukuba ukuchonga, ngokuguqula umbala zeendawana. Eyona njongo yale Uhlalutyo - ukuzimisela lobukho iimveliso ezahlukeneyo abasabela enzymatic kwi iisampuli zeemvavanyo.

Kuba efumanise antigen kunye antibodies isetyenziswa owahlukeneyo enzyme immunoassay, ekubeni inkqubo yalo lula ukwahlula amacandelo indlela kwaye kulibazise abanye babo kwisigaba esiqinileyo.

Kubalulekile ukuphawula iingenelo elandelayo yale sifundo laboratory:

• uvakalelo oluphezulu, hlolo olo lunokukhangela izinto nkqu inani elincinane molecule izinto;

• ukufeza olu hlalutyo Ungasebenzisa ubuncinane inani lezinto zokubhala uvavanyo;

• ukuba nokwenzeka ixesha elide ukugcina onke amacandelo ezidingekayo uhlalutyo;

• Easy ukwenza;

• Ubukho iziphumo negalelo nezibonwayo;

• kulingwa immunosorbent into automated kwinqanaba nganye indlela;

• iikiti ixabiso eliphantsi kakhulu asetyenziswa moshilongo.

Ezi eziluncedo sisebenzisa ngamandla Ifa kwiindawo ezahlukeneyo amayeza, kubandakanywa, ngenxa uxilongo iintsholongwane.

Inkqubo oluqhelekilyo immobilizing izilwa-buhlungu kunye nezakhi ukusabela adsorption, xa molekyuli ezahlukeneyo ziqhotyoshelwe kwisigaba eqinileyo (ngenxa nonxibelelwano ionic okanye hydrophobic okanye ukubunjwa iibhondi hydrogen).

Ndimele ndithi ukuba immunoassay enzyme lisetyenziswa iimeko ezimbini:

• ukuchonga ezisegazini iintsholongwane ze izifo ezahlukeneyo;

• ukuqinisekisa ubukho antibodies iantigens ezincinane zophendlo lwezifo (kufanele kuqatshelwe ukuba ELISA ukubona phantse naluphi na usuleleko zangasese).

Special istro plate yasetyenziswa ukwenza le serodiagnosis. Inemingxuma 96 emacaleni iantigens adsorbed. Emva ungeno kwi cwecwe wakha walinga na izilwa-cell iantigens Kuqhotyoshelwe efanelekileyo. Ukongeza esilandelayo lomzimba enombhalo ukuba imithombo immunoglobulins yabantu ubabonisa indlela ethile. Kule unyango ilandelayo serum chromogen (ngedayi) kuyahlukahlukana imibala zeendawana (phambi kwizakhiwo esihambelanayo "iantigens-unalo"). Kubalulekile ukuqaphela ukuba izinga umbala kisekiyo ngqo isixa omzimba.

Uhlalutyo ELISA egqibile yokulinganisa lexinene woqondo le ulwelo emithonjeni. Ngoko sebenzisa izixhobo ezikhethekileyo kunye neendlela beyenza ezinceda ukubala xi omzimba. Kufuneka kukhunjulwe ukuba yonke inkqubo uvavanyo ukuqhuba le hlalutyo unemilinganiselo yayo iziphawuli ezisemgangathweni (oku idla emiselweyo kwiziphumo, leyo zinikwa).

Ukusebenzisa ELISA ukuxilonga negcushuwa, hepatitis wentsholongwane egazini kunye HIV, kunye njengoko zifumaneka cytomegalovirus umzimba, chlamydia, i-herpes (iintlobo ezahlukeneyo). Olu hlahlelo kunceda ukuqinisekisa umgangatho hormone ukubona ngabamakishi somhlaza.

Apho ukuba bahlolelwe eMoscow? Namhlanje, kukho ezininzi kweelebhu ezizodwa, abonelela uluhlu olubanzi lweemvavanyo eziqhutyiweyo ze-serological. Indlela nceda udibane nogqirha we kwikliniki yengingqi (yabucala okanye umsebenzi, wena dveryaete). Kunye neziphumo zovavanyo kufuneka uye kwagqirha. Kwaye kuphulaphula izimvo zakhe malunga imfuneko naluphi na unyango.